Correlation of HbA[1c] with zinc and magnesium levels in patients with type 2 diabetes mellitus

To determine the plasma levels of Zn and Mg in Type 2 Diabetic patients with HbA[1c] >8% and <8% and to determine the correlation of Zn and Mg with HbA[1c]. A total population of 60 patients of Type 2 DM and 30 normal healthy control subjects were included in this study. Three ml of blood was collected in EDTA vacutainer tube [BD vacutainer] for determination of HbA[1c] and 6 ml blood was collected in EDTA vacutainer tube [BD vacutainer] for determination of Zn and Mg, HbA[1c] was performed on Bio Rad D10 hemoglobin testing system. Zn and Mg were measured on ContrAA 700 atomic absorption spectrophotometer. The age range of the patients and controls was 40 to 60 years. The mean age of the patients was 51.7 +/- 7.1 year and mean age of the control subjects was 46.7 +/- 7.2 years. Statistically significant differences were observed between levels of HbA[1c], Zn and Mg in patients and control groups [p < 0.05]. Mean Plasma Zn and Mg levels were low in Diabetic patients than in control group. We also found that diabetic patients with HbA[1c] >8% have lower levels of Zn and Mg as compared to diabetic patients with HbA[1c] <8%. A significant negative correlation of HbA[1c] with Zn and Mg was also observed. Type 2 Diabetes mellitus is associated with the deficiency of Zn and Mg. Patients with poor giycemic control have more severe deficiency of Zn and Mg than patients with good giycemic control. HbA[1c] levels correlate negatively with Zn and Mg in patients with type 2 diabetes mellitus

Effect of ascorbic acid on glycation level in diabetic condition

Diabetes is a serious metabolic disorder that results in significant morbidity and mortality. Diabetes is characterized by hyperglycaemia resulting in various short-term metabolic changes in lipid and protein metabolism and long-term irreversible vascular changes. In this study, effect of ascorbic acid on glycation was investigated. Experimental Study. Allied Hospital and National Hospital, Faisalabad. were performed by using normal and diabetic plasma. Samples were incubated for 5 weeks at 37 °C temperature and varying concentrations of glucose and vitamin C. Two glycation assays [Thioarbituric acid and Periodate] were used to measure and compare the glycation level. The results indicated that increase in glycation was observed from 1[st] to 3[rd] week of incubation and it was decreased after 5[th] week due to the formation of advanced glycation end products. With three concentrations of ascorbic acid variable responses were observed however, it was observed all three concentrations were responsible to increase glycation. Ascorbic acid will facilitate glycation in hyperglycaemia condition and Periodate borohydride proved itself more reliable and sensitive glycation assays than TEA test

Correlation of gestational lipid profile with neonatal birth weight

Lipids are heterogeneous group of water insoluble [hydrophobic] organic molecules that can be extracted from tissues by non polar solvent. Because of their property of insolubility in water they are mainly found in different compartments, mostly associated with membranes enclosing various cell organelles. The objective of this study was to find the correlation of gestational lipid profile with neonatal birth weight. Prospective analytical study of 100 Pakistani pregnant women [non diabetics and non-hypertensive] was carried out at Social Security Hospital, Lahore. Blood samples were collected from all the pregnant women during 24 - 36 weeks of pregnancy. Serum was separated by centrifugation process and stored in deep freezer at - 2 to -4[degree sign] C prior to processing and later on used for the estimation of lipid profile. The weight of each baby was done at the time of delivery. Positive correlation was found between material cholesterol levels and neonatal birth weight. Maternal lipid profile should be kept under check to control the weight of newborn baby

Comparison of different tratment regimens in infertile women with hyperprolactinaemia

A total of 70 patients with hyperprolactinaemic infertility were studied over a period of one year. Our goal was to compare two treatment regimens: bromocriptine plus placebo and bromocriptine plus clomiphene citrate in management of these patients. A double-blinded randomised, placebo-controlled trial was carried out. Ovulation/conception was achieved in a shorter time with greater safety and success rate in patients receiving combined therapy as compared to patients on bromocriptine alone